generative ai assisted technology tool Search Results


90
AstraZeneca ltd generative ai software reinvent
Generative Ai Software Reinvent, supplied by AstraZeneca ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
generative ai software reinvent - by Bioz Stars, 2026-06
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86
Grammarly Inc generative ai tools
Generative Ai Tools, supplied by Grammarly Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
generative ai tools - by Bioz Stars, 2026-06
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86
Pfizer Inc generative ai assisted technology tool
Generative Ai Assisted Technology Tool, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/generative ai assisted technology tool/product/Pfizer Inc
Average 86 stars, based on 1 article reviews
generative ai assisted technology tool - by Bioz Stars, 2026-06
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90
Omiq Inc omiq data analysis software
a BALB/c mice were injected subcutaneously with 1 × 10 5 CT26WT tumor cells. Treatment via intratumoral (i.t.) injections with vehicle (40% CDX) in PBS or with 4-OI (25 mg/kg/dose) in 40% CDX in PBS for 24 h prior challenge with VSVΔ51 expressing firefly luciferase (10 8 PFU) was given as indicated by arrows. Mice were euthanized seven days after the first VSVΔ51 injection for analysis. b Mean ( ± SEM) tumor growth of the groups followed the start of the treatment regimen ( n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and CDX-4-OI/VSV groups). Statistics on tumor volumes at day 8 indicate significance by one-way ANOVA followed by Šídák’s multiple comparisons test on tumor sizes at day 8. c Relative expression analyzed by RT-qPCR of RNA isolated from bulk tumor samples. n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and n = 4 CDX-4-OI/VSV groups (two samples excluded due to insufficient tumor material) d Flow cytometry data indicating the distribution of main T-cell populations within tumors upon different treatments analyzed by manual gating in FlowJo software. Mean ± SEM is displayed and compared per treatment group. Each data point represents one animal. n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 5 in CDX-VSV (one sample lost during acquisition), and n = 6 CDX-4-OI/VSV groups. e Expression intensity profile of myeloid markers on clustered live, CD45+, CD3− cells from merged samples ( n = 60, 3 different organs) to distinguish the regional expression of single myeloid markers. Relative expression is indicated by color where red indicates high expression and blue represents no expression within the cluster. <t>f</t> <t>Opt-SNE</t> cluster plots of live, CD45+, CD3− cells from indicated organs and treatment group ( n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and CDX-4-OI/VSV groups). Cell density is indicated by color where red indicates high density and blue indicates low density within the cluster. Analysis generated using <t>OMIQ</t> software. ( a ) was created using BioRender.com. Source data are provided as a Source Data file.
Omiq Data Analysis Software, supplied by Omiq Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
omiq data analysis software - by Bioz Stars, 2026-06
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86
Mendeley Ltd ai generative tools
a BALB/c mice were injected subcutaneously with 1 × 10 5 CT26WT tumor cells. Treatment via intratumoral (i.t.) injections with vehicle (40% CDX) in PBS or with 4-OI (25 mg/kg/dose) in 40% CDX in PBS for 24 h prior challenge with VSVΔ51 expressing firefly luciferase (10 8 PFU) was given as indicated by arrows. Mice were euthanized seven days after the first VSVΔ51 injection for analysis. b Mean ( ± SEM) tumor growth of the groups followed the start of the treatment regimen ( n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and CDX-4-OI/VSV groups). Statistics on tumor volumes at day 8 indicate significance by one-way ANOVA followed by Šídák’s multiple comparisons test on tumor sizes at day 8. c Relative expression analyzed by RT-qPCR of RNA isolated from bulk tumor samples. n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and n = 4 CDX-4-OI/VSV groups (two samples excluded due to insufficient tumor material) d Flow cytometry data indicating the distribution of main T-cell populations within tumors upon different treatments analyzed by manual gating in FlowJo software. Mean ± SEM is displayed and compared per treatment group. Each data point represents one animal. n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 5 in CDX-VSV (one sample lost during acquisition), and n = 6 CDX-4-OI/VSV groups. e Expression intensity profile of myeloid markers on clustered live, CD45+, CD3− cells from merged samples ( n = 60, 3 different organs) to distinguish the regional expression of single myeloid markers. Relative expression is indicated by color where red indicates high expression and blue represents no expression within the cluster. <t>f</t> <t>Opt-SNE</t> cluster plots of live, CD45+, CD3− cells from indicated organs and treatment group ( n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and CDX-4-OI/VSV groups). Cell density is indicated by color where red indicates high density and blue indicates low density within the cluster. Analysis generated using <t>OMIQ</t> software. ( a ) was created using BioRender.com. Source data are provided as a Source Data file.
Ai Generative Tools, supplied by Mendeley Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
ai generative tools - by Bioz Stars, 2026-06
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86
Varian Medical ai based auto segmentation software rtmindprocess
a BALB/c mice were injected subcutaneously with 1 × 10 5 CT26WT tumor cells. Treatment via intratumoral (i.t.) injections with vehicle (40% CDX) in PBS or with 4-OI (25 mg/kg/dose) in 40% CDX in PBS for 24 h prior challenge with VSVΔ51 expressing firefly luciferase (10 8 PFU) was given as indicated by arrows. Mice were euthanized seven days after the first VSVΔ51 injection for analysis. b Mean ( ± SEM) tumor growth of the groups followed the start of the treatment regimen ( n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and CDX-4-OI/VSV groups). Statistics on tumor volumes at day 8 indicate significance by one-way ANOVA followed by Šídák’s multiple comparisons test on tumor sizes at day 8. c Relative expression analyzed by RT-qPCR of RNA isolated from bulk tumor samples. n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and n = 4 CDX-4-OI/VSV groups (two samples excluded due to insufficient tumor material) d Flow cytometry data indicating the distribution of main T-cell populations within tumors upon different treatments analyzed by manual gating in FlowJo software. Mean ± SEM is displayed and compared per treatment group. Each data point represents one animal. n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 5 in CDX-VSV (one sample lost during acquisition), and n = 6 CDX-4-OI/VSV groups. e Expression intensity profile of myeloid markers on clustered live, CD45+, CD3− cells from merged samples ( n = 60, 3 different organs) to distinguish the regional expression of single myeloid markers. Relative expression is indicated by color where red indicates high expression and blue represents no expression within the cluster. <t>f</t> <t>Opt-SNE</t> cluster plots of live, CD45+, CD3− cells from indicated organs and treatment group ( n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and CDX-4-OI/VSV groups). Cell density is indicated by color where red indicates high density and blue indicates low density within the cluster. Analysis generated using <t>OMIQ</t> software. ( a ) was created using BioRender.com. Source data are provided as a Source Data file.
Ai Based Auto Segmentation Software Rtmindprocess, supplied by Varian Medical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
ai based auto segmentation software rtmindprocess - by Bioz Stars, 2026-06
86/100 stars
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90
RenderX Inc xsl•fo renderx
a BALB/c mice were injected subcutaneously with 1 × 10 5 CT26WT tumor cells. Treatment via intratumoral (i.t.) injections with vehicle (40% CDX) in PBS or with 4-OI (25 mg/kg/dose) in 40% CDX in PBS for 24 h prior challenge with VSVΔ51 expressing firefly luciferase (10 8 PFU) was given as indicated by arrows. Mice were euthanized seven days after the first VSVΔ51 injection for analysis. b Mean ( ± SEM) tumor growth of the groups followed the start of the treatment regimen ( n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and CDX-4-OI/VSV groups). Statistics on tumor volumes at day 8 indicate significance by one-way ANOVA followed by Šídák’s multiple comparisons test on tumor sizes at day 8. c Relative expression analyzed by RT-qPCR of RNA isolated from bulk tumor samples. n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and n = 4 CDX-4-OI/VSV groups (two samples excluded due to insufficient tumor material) d Flow cytometry data indicating the distribution of main T-cell populations within tumors upon different treatments analyzed by manual gating in FlowJo software. Mean ± SEM is displayed and compared per treatment group. Each data point represents one animal. n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 5 in CDX-VSV (one sample lost during acquisition), and n = 6 CDX-4-OI/VSV groups. e Expression intensity profile of myeloid markers on clustered live, CD45+, CD3− cells from merged samples ( n = 60, 3 different organs) to distinguish the regional expression of single myeloid markers. Relative expression is indicated by color where red indicates high expression and blue represents no expression within the cluster. <t>f</t> <t>Opt-SNE</t> cluster plots of live, CD45+, CD3− cells from indicated organs and treatment group ( n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and CDX-4-OI/VSV groups). Cell density is indicated by color where red indicates high density and blue indicates low density within the cluster. Analysis generated using <t>OMIQ</t> software. ( a ) was created using BioRender.com. Source data are provided as a Source Data file.
Xsl•Fo Renderx, supplied by RenderX Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
xsl•fo renderx - by Bioz Stars, 2026-06
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86
Indica Labs 539 halo software
a BALB/c mice were injected subcutaneously with 1 × 10 5 CT26WT tumor cells. Treatment via intratumoral (i.t.) injections with vehicle (40% CDX) in PBS or with 4-OI (25 mg/kg/dose) in 40% CDX in PBS for 24 h prior challenge with VSVΔ51 expressing firefly luciferase (10 8 PFU) was given as indicated by arrows. Mice were euthanized seven days after the first VSVΔ51 injection for analysis. b Mean ( ± SEM) tumor growth of the groups followed the start of the treatment regimen ( n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and CDX-4-OI/VSV groups). Statistics on tumor volumes at day 8 indicate significance by one-way ANOVA followed by Šídák’s multiple comparisons test on tumor sizes at day 8. c Relative expression analyzed by RT-qPCR of RNA isolated from bulk tumor samples. n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and n = 4 CDX-4-OI/VSV groups (two samples excluded due to insufficient tumor material) d Flow cytometry data indicating the distribution of main T-cell populations within tumors upon different treatments analyzed by manual gating in FlowJo software. Mean ± SEM is displayed and compared per treatment group. Each data point represents one animal. n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 5 in CDX-VSV (one sample lost during acquisition), and n = 6 CDX-4-OI/VSV groups. e Expression intensity profile of myeloid markers on clustered live, CD45+, CD3− cells from merged samples ( n = 60, 3 different organs) to distinguish the regional expression of single myeloid markers. Relative expression is indicated by color where red indicates high expression and blue represents no expression within the cluster. <t>f</t> <t>Opt-SNE</t> cluster plots of live, CD45+, CD3− cells from indicated organs and treatment group ( n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and CDX-4-OI/VSV groups). Cell density is indicated by color where red indicates high density and blue indicates low density within the cluster. Analysis generated using <t>OMIQ</t> software. ( a ) was created using BioRender.com. Source data are provided as a Source Data file.
539 Halo Software, supplied by Indica Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/539 halo software/product/Indica Labs
Average 86 stars, based on 1 article reviews
539 halo software - by Bioz Stars, 2026-06
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86
Databricks Inc llm tools
a BALB/c mice were injected subcutaneously with 1 × 10 5 CT26WT tumor cells. Treatment via intratumoral (i.t.) injections with vehicle (40% CDX) in PBS or with 4-OI (25 mg/kg/dose) in 40% CDX in PBS for 24 h prior challenge with VSVΔ51 expressing firefly luciferase (10 8 PFU) was given as indicated by arrows. Mice were euthanized seven days after the first VSVΔ51 injection for analysis. b Mean ( ± SEM) tumor growth of the groups followed the start of the treatment regimen ( n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and CDX-4-OI/VSV groups). Statistics on tumor volumes at day 8 indicate significance by one-way ANOVA followed by Šídák’s multiple comparisons test on tumor sizes at day 8. c Relative expression analyzed by RT-qPCR of RNA isolated from bulk tumor samples. n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and n = 4 CDX-4-OI/VSV groups (two samples excluded due to insufficient tumor material) d Flow cytometry data indicating the distribution of main T-cell populations within tumors upon different treatments analyzed by manual gating in FlowJo software. Mean ± SEM is displayed and compared per treatment group. Each data point represents one animal. n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 5 in CDX-VSV (one sample lost during acquisition), and n = 6 CDX-4-OI/VSV groups. e Expression intensity profile of myeloid markers on clustered live, CD45+, CD3− cells from merged samples ( n = 60, 3 different organs) to distinguish the regional expression of single myeloid markers. Relative expression is indicated by color where red indicates high expression and blue represents no expression within the cluster. <t>f</t> <t>Opt-SNE</t> cluster plots of live, CD45+, CD3− cells from indicated organs and treatment group ( n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and CDX-4-OI/VSV groups). Cell density is indicated by color where red indicates high density and blue indicates low density within the cluster. Analysis generated using <t>OMIQ</t> software. ( a ) was created using BioRender.com. Source data are provided as a Source Data file.
Llm Tools, supplied by Databricks Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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90
DelStar Technologies delstar ai tool
a BALB/c mice were injected subcutaneously with 1 × 10 5 CT26WT tumor cells. Treatment via intratumoral (i.t.) injections with vehicle (40% CDX) in PBS or with 4-OI (25 mg/kg/dose) in 40% CDX in PBS for 24 h prior challenge with VSVΔ51 expressing firefly luciferase (10 8 PFU) was given as indicated by arrows. Mice were euthanized seven days after the first VSVΔ51 injection for analysis. b Mean ( ± SEM) tumor growth of the groups followed the start of the treatment regimen ( n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and CDX-4-OI/VSV groups). Statistics on tumor volumes at day 8 indicate significance by one-way ANOVA followed by Šídák’s multiple comparisons test on tumor sizes at day 8. c Relative expression analyzed by RT-qPCR of RNA isolated from bulk tumor samples. n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and n = 4 CDX-4-OI/VSV groups (two samples excluded due to insufficient tumor material) d Flow cytometry data indicating the distribution of main T-cell populations within tumors upon different treatments analyzed by manual gating in FlowJo software. Mean ± SEM is displayed and compared per treatment group. Each data point represents one animal. n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 5 in CDX-VSV (one sample lost during acquisition), and n = 6 CDX-4-OI/VSV groups. e Expression intensity profile of myeloid markers on clustered live, CD45+, CD3− cells from merged samples ( n = 60, 3 different organs) to distinguish the regional expression of single myeloid markers. Relative expression is indicated by color where red indicates high expression and blue represents no expression within the cluster. <t>f</t> <t>Opt-SNE</t> cluster plots of live, CD45+, CD3− cells from indicated organs and treatment group ( n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and CDX-4-OI/VSV groups). Cell density is indicated by color where red indicates high density and blue indicates low density within the cluster. Analysis generated using <t>OMIQ</t> software. ( a ) was created using BioRender.com. Source data are provided as a Source Data file.
Delstar Ai Tool, supplied by DelStar Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/delstar ai tool/product/DelStar Technologies
Average 90 stars, based on 1 article reviews
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86
Dotmatics Limited omiq software
a BALB/c mice were injected subcutaneously with 1 × 10 5 CT26WT tumor cells. Treatment via intratumoral (i.t.) injections with vehicle (40% CDX) in PBS or with 4-OI (25 mg/kg/dose) in 40% CDX in PBS for 24 h prior challenge with VSVΔ51 expressing firefly luciferase (10 8 PFU) was given as indicated by arrows. Mice were euthanized seven days after the first VSVΔ51 injection for analysis. b Mean ( ± SEM) tumor growth of the groups followed the start of the treatment regimen ( n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and CDX-4-OI/VSV groups). Statistics on tumor volumes at day 8 indicate significance by one-way ANOVA followed by Šídák’s multiple comparisons test on tumor sizes at day 8. c Relative expression analyzed by RT-qPCR of RNA isolated from bulk tumor samples. n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and n = 4 CDX-4-OI/VSV groups (two samples excluded due to insufficient tumor material) d Flow cytometry data indicating the distribution of main T-cell populations within tumors upon different treatments analyzed by manual gating in FlowJo software. Mean ± SEM is displayed and compared per treatment group. Each data point represents one animal. n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 5 in CDX-VSV (one sample lost during acquisition), and n = 6 CDX-4-OI/VSV groups. e Expression intensity profile of myeloid markers on clustered live, CD45+, CD3− cells from merged samples ( n = 60, 3 different organs) to distinguish the regional expression of single myeloid markers. Relative expression is indicated by color where red indicates high expression and blue represents no expression within the cluster. <t>f</t> <t>Opt-SNE</t> cluster plots of live, CD45+, CD3− cells from indicated organs and treatment group ( n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and CDX-4-OI/VSV groups). Cell density is indicated by color where red indicates high density and blue indicates low density within the cluster. Analysis generated using <t>OMIQ</t> software. ( a ) was created using BioRender.com. Source data are provided as a Source Data file.
Omiq Software, supplied by Dotmatics Limited, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/omiq software/product/Dotmatics Limited
Average 86 stars, based on 1 article reviews
omiq software - by Bioz Stars, 2026-06
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90
DEEPHEALTH INC ai breast density estimation tool
a BALB/c mice were injected subcutaneously with 1 × 10 5 CT26WT tumor cells. Treatment via intratumoral (i.t.) injections with vehicle (40% CDX) in PBS or with 4-OI (25 mg/kg/dose) in 40% CDX in PBS for 24 h prior challenge with VSVΔ51 expressing firefly luciferase (10 8 PFU) was given as indicated by arrows. Mice were euthanized seven days after the first VSVΔ51 injection for analysis. b Mean ( ± SEM) tumor growth of the groups followed the start of the treatment regimen ( n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and CDX-4-OI/VSV groups). Statistics on tumor volumes at day 8 indicate significance by one-way ANOVA followed by Šídák’s multiple comparisons test on tumor sizes at day 8. c Relative expression analyzed by RT-qPCR of RNA isolated from bulk tumor samples. n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and n = 4 CDX-4-OI/VSV groups (two samples excluded due to insufficient tumor material) d Flow cytometry data indicating the distribution of main T-cell populations within tumors upon different treatments analyzed by manual gating in FlowJo software. Mean ± SEM is displayed and compared per treatment group. Each data point represents one animal. n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 5 in CDX-VSV (one sample lost during acquisition), and n = 6 CDX-4-OI/VSV groups. e Expression intensity profile of myeloid markers on clustered live, CD45+, CD3− cells from merged samples ( n = 60, 3 different organs) to distinguish the regional expression of single myeloid markers. Relative expression is indicated by color where red indicates high expression and blue represents no expression within the cluster. <t>f</t> <t>Opt-SNE</t> cluster plots of live, CD45+, CD3− cells from indicated organs and treatment group ( n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and CDX-4-OI/VSV groups). Cell density is indicated by color where red indicates high density and blue indicates low density within the cluster. Analysis generated using <t>OMIQ</t> software. ( a ) was created using BioRender.com. Source data are provided as a Source Data file.
Ai Breast Density Estimation Tool, supplied by DEEPHEALTH INC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a BALB/c mice were injected subcutaneously with 1 × 10 5 CT26WT tumor cells. Treatment via intratumoral (i.t.) injections with vehicle (40% CDX) in PBS or with 4-OI (25 mg/kg/dose) in 40% CDX in PBS for 24 h prior challenge with VSVΔ51 expressing firefly luciferase (10 8 PFU) was given as indicated by arrows. Mice were euthanized seven days after the first VSVΔ51 injection for analysis. b Mean ( ± SEM) tumor growth of the groups followed the start of the treatment regimen ( n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and CDX-4-OI/VSV groups). Statistics on tumor volumes at day 8 indicate significance by one-way ANOVA followed by Šídák’s multiple comparisons test on tumor sizes at day 8. c Relative expression analyzed by RT-qPCR of RNA isolated from bulk tumor samples. n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and n = 4 CDX-4-OI/VSV groups (two samples excluded due to insufficient tumor material) d Flow cytometry data indicating the distribution of main T-cell populations within tumors upon different treatments analyzed by manual gating in FlowJo software. Mean ± SEM is displayed and compared per treatment group. Each data point represents one animal. n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 5 in CDX-VSV (one sample lost during acquisition), and n = 6 CDX-4-OI/VSV groups. e Expression intensity profile of myeloid markers on clustered live, CD45+, CD3− cells from merged samples ( n = 60, 3 different organs) to distinguish the regional expression of single myeloid markers. Relative expression is indicated by color where red indicates high expression and blue represents no expression within the cluster. f Opt-SNE cluster plots of live, CD45+, CD3− cells from indicated organs and treatment group ( n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and CDX-4-OI/VSV groups). Cell density is indicated by color where red indicates high density and blue indicates low density within the cluster. Analysis generated using OMIQ software. ( a ) was created using BioRender.com. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Octyl itaconate enhances VSVΔ51 oncolytic virotherapy by multitarget inhibition of antiviral and inflammatory pathways

doi: 10.1038/s41467-024-48422-x

Figure Lengend Snippet: a BALB/c mice were injected subcutaneously with 1 × 10 5 CT26WT tumor cells. Treatment via intratumoral (i.t.) injections with vehicle (40% CDX) in PBS or with 4-OI (25 mg/kg/dose) in 40% CDX in PBS for 24 h prior challenge with VSVΔ51 expressing firefly luciferase (10 8 PFU) was given as indicated by arrows. Mice were euthanized seven days after the first VSVΔ51 injection for analysis. b Mean ( ± SEM) tumor growth of the groups followed the start of the treatment regimen ( n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and CDX-4-OI/VSV groups). Statistics on tumor volumes at day 8 indicate significance by one-way ANOVA followed by Šídák’s multiple comparisons test on tumor sizes at day 8. c Relative expression analyzed by RT-qPCR of RNA isolated from bulk tumor samples. n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and n = 4 CDX-4-OI/VSV groups (two samples excluded due to insufficient tumor material) d Flow cytometry data indicating the distribution of main T-cell populations within tumors upon different treatments analyzed by manual gating in FlowJo software. Mean ± SEM is displayed and compared per treatment group. Each data point represents one animal. n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 5 in CDX-VSV (one sample lost during acquisition), and n = 6 CDX-4-OI/VSV groups. e Expression intensity profile of myeloid markers on clustered live, CD45+, CD3− cells from merged samples ( n = 60, 3 different organs) to distinguish the regional expression of single myeloid markers. Relative expression is indicated by color where red indicates high expression and blue represents no expression within the cluster. f Opt-SNE cluster plots of live, CD45+, CD3− cells from indicated organs and treatment group ( n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and CDX-4-OI/VSV groups). Cell density is indicated by color where red indicates high density and blue indicates low density within the cluster. Analysis generated using OMIQ software. ( a ) was created using BioRender.com. Source data are provided as a Source Data file.

Article Snippet: Opt-SNE plots were generated using standard settings in OMIQ data analysis software (Omiq, Inc. www.omiq.ai ) .

Techniques: Injection, Expressing, Luciferase, Quantitative RT-PCR, Isolation, Flow Cytometry, Software, Generated